[HTML][HTML] Mutation of residue 33 of human equilibrative nucleoside transporters 1 and 2 alters sensitivity to inhibition of transport by dilazep and dipyridamole
F Visser, MF Vickers, AML Ng, SA Baldwin… - Journal of Biological …, 2002 - ASBMB
Human equilibrative nucleoside transporters (hENT) 1 and 2 differ in that hENT1 is inhibited
by nanomolar concentrations of dipyridamole and dilazep, whereas hENT2 is 2 and 3 orders
of magnitude less sensitive, respectively. When a yeast expression plasmid containing the
hENT1 cDNA was randomly mutated and screened by phenotypic complementation in
Saccharomyces cerevisiae to identify mutants with reduced sensitivity to dilazep, clones with
a point mutation that converted Met 33 to Ile (hENT1-M33I) were obtained. Characterization …
by nanomolar concentrations of dipyridamole and dilazep, whereas hENT2 is 2 and 3 orders
of magnitude less sensitive, respectively. When a yeast expression plasmid containing the
hENT1 cDNA was randomly mutated and screened by phenotypic complementation in
Saccharomyces cerevisiae to identify mutants with reduced sensitivity to dilazep, clones with
a point mutation that converted Met 33 to Ile (hENT1-M33I) were obtained. Characterization …
